Oct, 1, 2023

Vol.30 No.2, pp. 84-88

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Review

  • Korean Journal of Biological Psychiatry
  • Volume 3(2); 1996
  • Article

Review

Korean Journal of Biological Psychiatry 1996;3(2):181-90. Published online: Feb, 1, 1996

Abstract PDF

Quantitative RT-PCR for Measuring C-fos Gene Expression in Rat Brain after ECS

  • Byung-Hwan Yang, MD1;Jei-Wook Lee, MD2;Eung-Chul Park, MD2;Jae-Hak Yu, MD3;Goang-Won Cho, MSc4;Bo-Gee Yang, MD4; and Young-Gyu Chai, PhD4;
    1;Department of Neuropsychiatry, School of Medicine & The Mental Health Research Institute, Hanyang University, Seoul, 2;Department of Neuropsychiatry, School of Medicine, Hanyang University, Seoul, Korea, 3;Department of Neuropsychiatry, Stony Brook Hospital, NewYor, U.S.A., 4;Department of Biochimistry, College of Science, Hanyang University, Ansan, Korea
Abstract

To clarify the mechanism of action of electroconvulsive shock(ECS) in respect to molecular biology, and to detect the quantitative amount of change of c-fos gene expression after ECS in the rat's brain, the authors obtained brain specimens from the striatum, cerebral cortex, hippocampus, and cerebellum. Each brain was removed within 30min. after ECS(130V, 0.5sec) and ECS-sham. Then we performed RT-PCR. The results are 1) ECS was found to affect the expression of immediate early genes. 2) The cerebral cortex and hippocampus was more influenced by ECS than in the cerebellum and striatum. From these results, we can suggest that ECS is related to the mechanism of cognition, mood, memory which is correlated to cerebral cortex and hippocampus.

Keywords C-fos expression;ECS;Hippocampus;Cerebral cortex;RT-PCT.