Oct, 1, 2023

Vol.30 No.2, pp. 84-88

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Review

  • Korean Journal of Biological Psychiatry
  • Volume 4(2); 1997
  • Article

Review

Korean Journal of Biological Psychiatry 1997;4(2):211-7. Published online: Feb, 1, 1997

Abstract PDF Full Text

Cell Death Induced by Ethanol：Prevention of Cell Death by the bcl-2 Proto-Oncogene

  • Eun-Jeong Lim, MSc1;Kyoung-Ja Hong, PhD1;Byung-Hwan Yang, MD2; and Young-Gyu Chai, PhD2;
    1;Department of Biochemistry and Molecular Biology, College of Science, Hanyang University, Ansan, 2;Department of Neuropsychiatry, College of Medicine & The Mental Health Research Institute, Hanyang University, Seoul, Korea
Abstract

The Bcl-2 protein has been shown to block apoptosis induced by a variety of stimuli. We have performed the experiments which cell death can be blocked by the bcl-2 proto-oncogene under moderate(50-100mM) or high ethanol treatment(400-600mM). As a result of morphological changes, and MTT assay, cell death was blocked by Bcl-2 under 100mM ethanol. However, the results of DNA fragmentation and RT-PCR(ICE, and CPP32), immunoblotting(CPP32, and PARP) for SK-pcDNA3 cells(vector only) and SK-Bcl-2 cells(stably expressed bcl-2 gene) were showen to be no significant differences between two cell lines. These results suggested that cell death induced by ethanol was not followed by apoptosis mechanism, and was blocked by the bcl-2 proto-oncogene with moderate ethanol.

Keywords Bcl-2;Ethanol;Apoptosis;ICE;CPP32;PARP.